LOCUS       40924_4075       10000 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pADNpcDNA3.1 KanR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Ambati S, Yu P, McKinney EC, Kandasamy MK, Hartzell D, Baile CA, 
            Meagher RB.
  TITLE     Adipocyte nuclei captured from VAT and SAT
  JOURNAL   BMC Obes 3, 35 (2016)
  PUBMED    27462403
REFERENCE   2  (bases 1 to 10000)
  AUTHORS   Ambati S, Yu P, McKinney EC, Kandasamy MK, Hartzell D, Baile CA, 
            Meagher RB.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2016) Genetics, University of Georgia, 120 East 
            Green Street, Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 10000)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10000)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Obes 3,
            35 (2016)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAY-2016) Genetics, University of Georgia, 120 East Green 
            Street, Athens, GA 30602, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      37..5486
                     /label=ADNp*
                     /note="ADNp*"
                     /regulatory_class="promoter"
     misc_feature    2820..2825
                     /label=AgeI site
                     /note="AgeI site"
     misc_feature    5487..5548
                     /label=multiple cloning site
                     /note="multiple cloning site"
     polyA_signal    5580..5804
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5850..6278
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6292..6621
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             6688..7479
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    7656..7789
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7826..7842)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7850..7866
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7874..7904)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7919..7940)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8248..8833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9007..9864)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9865..9969)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctaag cggccgcgat tccggaggta ccgctagtgt atgcaatggt
       61 gtcagcgttt ggaagctgat tatgggatgg atccctggat atggcaatca ctagatggtc
      121 tatccttttg tcacagctcc aaattttgtc tctgtaactc cttccatggg tgttttgttc
      181 ccatttctaa gaaggggcaa agtgtccaca ctttggtctt tgttcttctt gagtttcatg
      241 cgtttagcaa attgtatctt atatcttggg tattctaaat gtctgggcta atatccactt
      301 atcagtgagt acatattgtg tgagttcctt tgtgattggg ttacctcact caggatgatg
      361 ccctccaggt ctatccattt gcctaggaat ttcataaatt cattcttttt aatagctgag
      421 tagtactcca ttgtgtaaat gtaccacatt tctgtatcca ttcctctgtt gaggggcatc
      481 tgggttcttt ccagcttctg gctattataa ataaggctgc tatgaacata gtggagcatg
      541 tgtccttctt actggttggg acatcttctg gatatatgcc caggagaggt attgtgggat
      601 cctccggtag tactatgtcc aattttctga ggaaccgcca gactgatttc cagagtggtt
      661 gtacaagctt gcaatcccac caacaatgga ggagtattcc tctttctcca catcctcgcc
      721 agcatctgct gtcacctgaa tttttgatct tagccattct gactggtgtg aggtggaatc
      781 tcagggttgt tttgatttgc atttccctga aggtccctac tatttatcta acattttgaa
      841 tgcaacattc tttcctttgt acatagtcat atgccatgtg cattagtaat ctgctataat
      901 acagacccaa cgatgagacg actcagacaa aagagtgtat ttgtatctct tgtgacatct
      961 aggatatagg ggaagaaaca ctccacacag ttatgcaggg ctctctgttt tgttttgttt
     1021 ttaaagattt attttatttc atttatttta tgtatataaa atacatgaaa tgagtacact
     1081 gtagctatac tgatggttgt gagccatcat gtggtttctg ggaattagaa ttcagggcct
     1141 ctgcttgctc tagttggctc cagttgctcc agtctaaaga tttatttatt attagatcta
     1201 agtacattgt agctgtcttc aaacacacca gaagagggca taggatccca ttacagatgg
     1261 ttgtgagcca ccatgtggtt gctgggattt gaactcagga cctctggaag agcagtcagt
     1321 gctcttaact gctgagccat ctctccagcc cttagcctgc tctcttatat catctatgag
     1381 cacctgtcta tggtaacagc accacaatgg gctggacctt acctcctcaa tcatcaagca
     1441 agaaaacgtc ccataggctt gcccacaggg cagtctggtg ggagcatttc cccaactgat
     1501 gttctctccc caaatgactt atctgataca ctggccttcg gagaaaagca agtcaacacc
     1561 tcattgtgat cccattttac ccagtcagaa tgtcataaga aaacaaataa cattttcata
     1621 agaaaataaa caacaataca ttctgtcaag gatgtagaca gatgggatgc acttgattca
     1681 ctgctggtgg gaatgataac tagtccaatc tcagtggaag tcagtctgga gtgttctcag
     1741 aaaatgaaaa aatagctggg cagtggtggc agatgccttt aatcccagca cttgggaggc
     1801 agaggcaggt ggatttctga gtttgaggcc agccttgtct acagagtgag ttccaggaca
     1861 gccaaggcta cacagagaaa ccctgtctca aaaaaccaaa aaaaagccgg acgtggtggc
     1921 gcacgccttt aatcccagca ctcgggaggc agaggcaggc ggatttctga gttcgaggcc
     1981 agcctggtct acagagtgag tttcaggaca gccagggcta cacagagaaa ccctgtctcg
     2041 aaaaaccaaa aaaaaaaaaa aaaaaaaaaa aaggaagtga agaatagctg tggataacac
     2101 agcttttctt ttctttatat ttcaggcatc agggatagag gtcaggtgtc aatgtaaatc
     2161 actctaccac tgagttacac ttctaaatta aagacatttt tcctttcttg aatgcccaaa
     2221 aatttggagt actgcaggac aggctcactc ttctgcagtt agtgttatgt ctcaaaattt
     2281 gatttgaggt ttagttgttt cagtaaattg tctttttaaa gtcatgacta aacaataata
     2341 tacagtgaaa tttaaccaca ttcaggaaac actgaggaaa ctcaggcctg tggggaaatc
     2401 tggccttttg tttaagtatc tcacttcgca tgtaggcgag aattgagatg tgaacatgag
     2461 ctcctgaagg aaaggaccac gtttctgtgg ctgcttcact ctgggcagta acacatctgt
     2521 ccaggtaaac ccagccttgc tgaacgtcaa tctcagcact tgggaggcag aggtgagagt
     2581 ttgaggccag tctgatctat agagcaagtt ccaggatagc ttgggctaca ctgaaaaaac
     2641 actgtcttga aaaacaaaac aaaacaaaac aaaacaaaac aaaacaaaac aaaacaccaa
     2701 aaaccaaaaa agaaaaaaaa aaacaggaaa agaaaagaaa aaaatcaaaa taaaacaaag
     2761 ccaaaaacaa agcaaaaaaa aaaaccaccc agcaaaaaac caaaccgcct agcctcaaga
     2821 ccggtgtggt tgaatgtttt tcacttctag tcgctaagca agtgtgtgtt tttacacaat
     2881 gccctctgtg gtgagtggcg gattcccctg agagttcacc aaatgatagg ctttcacaat
     2941 gctcccgggt gtctaccaga cccagcaaag tattgatgtg gttttggggt gaaagtcact
     3001 ctgtcttgtg caatagttag aatctgctga aaccagcagt gttcctatat gggacagggg
     3061 tccagagcta acccggaggc tataactgag cagaggtgaa gaccacgagg cattggggag
     3121 cgtatgccct ttgtggtcag agagatctag cttcgtgcct tgggtctgtg tctctccctc
     3181 ttactggctt ctggcttctt cattaagtgg gagacaacca caggtatctg tatgggaaga
     3241 ctcgactacc ccttgactca acattgcttg ttacttactt tgtacaagat actacttagt
     3301 ctaggggtta tggagcataa cctcaagtag gtaaagcccc tgctccagcg tgtttgcatt
     3361 ccagtaagaa gcgaaagaca gtaacacaca tacaaaataa gtaagaaaat gcaacaacag
     3421 caacaacaac aacacacaca cacaaagtaa gcaaaacgct aagggaaaga tagagagtga
     3481 tacagctttg agttgctgta gttcttctct ctcctttgct tcatacagtt tgcttgggaa
     3541 gtgtccaggg ccatggggtc acaactaaca gcccttggaa atgagcttgt gtccttaatc
     3601 ttcatgacct aacgtgattt ctctagaaac atcagtgcat taacaggaag acaagatgga
     3661 agatcatatt ttggctctcc ttccttggtg ggttgacact gctggtccta tccactagta
     3721 aaagcatgac tcttaggctc tgtgtggcca gtggaaggtg gcagttggag gaagcagatg
     3781 cttggccagc ctttgcctgg gagcagtcta gctctgagtg tcttattgga gcagctgctg
     3841 gcatccagag ttctttttgg attcacgatt taattcaaaa gctttgtgct cccgagaatc
     3901 agctctggtc tttcaaaaat aagatgtgag tccgccgaga ggctcccaag gtattgcctt
     3961 gccaactgca agccttttag gagcagttta gtgagtggtg actgctagtt gcagttggct
     4021 gttagcccag agctaataat agatagaaaa ggtatatact taaggagtct ggaaactgag
     4081 gtttatctac tcacagaaaa tgagtttcta aaaaactagc ttgaaactta cccagaaaaa
     4141 tcttagaaca tggttctcca atgtcaaggt aagtgttctg tgacactggg cttgaattat
     4201 gtagggacca cagattttag aatttggacc cctgaacttg cttcacaccc caccaggaac
     4261 cttcctgtac aacagccctc agaattcatc tacatggtct tttctcagta tgggatccgg
     4321 tctagcaagt ggagcacacc ttctattgct taaagatttg tttatgtata tgggtatttt
     4381 ggctgcatgc atatttgcac accaaaagaa ggcagcggat cccatggaat tactgtgggt
     4441 gctgggaatt gaactcagga cctctggaag aatagccagt gctcttaacc actgagccat
     4501 gcctgcagtc catctatttt ttattctagt acagcccctc ttcattctta ctgaaatagt
     4561 aatgcctgaa ccacacagct tcacatttag ttacaaagaa agagtgggag tatcatgtga
     4621 caattagtgt tgttgactct ccaggacaaa cttatgggaa agggaggtct cctgacccct
     4681 gaacaatcat tttacttgag gataattttc attgcactca gaaacatgct gaattattgt
     4741 ccttaccctt gccccatctc ttgctctggt agagaatggc caaagcctgg aaacaggatg
     4801 gcttgacaga agctctactt ggcttcccag acccaagctg gattaaacca ggttccctaa
     4861 ggagtcttaa ggcagctgcc aggagcaagg ggcccactca ttggctattg gccttgactg
     4921 ggttggccaa tggtaagctg gggtctgcct gtccccatga gtaccagact aatgagacct
     4981 ggccactttc tcctcatttc tgtctgtacg attgtcagtg gatctgacga caccaaaagg
     5041 taagaacaat tctatattct cggctggctg ggtatgaatg cagaatccca cttgggctgt
     5101 gttcagattt tgctctctgc agcagtgtga aggtagatgt tgagaaaact caggccttgg
     5161 aaacatggtt ggggcagata agctttgggg cttttcttta actcttcaaa gctctaagaa
     5221 agaaacaata acctttcggt ccacaagctt gagcctaacg gtgtctgttc acagcagtaa
     5281 aaccccaact aagacactga tgaagacctc ctgggagagt gagggctggg tagccactga
     5341 aggctctctg ggagaggcga gtatgtagat gcagatcttt ggagtggatt ccacttagct
     5401 atataggaca tgatgcaggt cctgattgga tgtgccatgt gagtctgcct ttcccatgac
     5461 tattcacctg tcaatttcag ggctcaacat gttttattaa ttaaaccgag ctcagaggat
     5521 atccagctag cacagtgggc cctctagagg gcccgtttaa acccgctgat cagcctcgac
     5581 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     5641 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     5701 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     5761 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     5821 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     5881 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5941 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6001 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6061 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6121 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6181 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6241 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     6301 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6361 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6421 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6481 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6541 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     6601 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     6661 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     6721 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     6781 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     6841 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     6901 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     6961 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     7021 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     7081 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     7141 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     7201 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     7261 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     7321 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     7381 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     7441 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     7501 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     7561 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     7621 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     7681 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7741 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     7801 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7861 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7921 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7981 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8041 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8101 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8161 gaaagaacat gacctgcggc cgctcctcat gtgagcaaaa ggccagcaaa aggccaggaa
     8221 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8281 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8341 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8401 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8461 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8521 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8581 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8641 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8701 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8761 caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8821 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8881 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8941 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9001 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9061 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9121 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9181 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9241 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9301 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9361 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9421 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9481 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9541 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9601 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9661 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9721 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9781 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9841 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9901 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9961 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
